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> Pepsin porcine ca. 15 milliAnson units/mg 2xcryst., lyophilised

Pepsin porcine ca. 15 milliAnson units/mg 2xcryst., lyophilised

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Cat# 31820.01

Size : 1G

Brand : SERVA Electrophoresis

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Phone : +1 850 650 7790

(Pepsin A)
EC 3.4.23.1 • Mr ca. 36 000 • CAS [9001-75-6]

For the specific digestion of proteins prior to mass spectrometric analyses and further applications in protein research. Endoprotease, cleaves carboxyterminally at the position of the aromatic amino acids phenylalanine, tyrosine and tryptophan. Can also be used in combination with other proteases.

Unit definition: 1 milliAnson unit is equivalent to 1 µmole of Folin-positive amino acids calculated as tyrosine, released from denatured hemoglobin per minute at 37 °C, pH 2.0.
Activity in other units: ca. 0.015 PUHb/mg ("Pepsin-Unit" according to Anson (1, 2). Expressed in millimole tyrosine, therefore 1 PUHb = 1000 milliAnson units.)


References:
  1. Anson, M.L. (1938) J. Gen. Physiol. 22, 79-98
  2. Ryle, A.P. (1984) Methods of Enzymatic Analysis, (Bergmeyer, H.U. ed.) 3rd Ed. vol. 5, 223-38
  3. Northrop, J.H. et al. (1948) Crystalline Enzymes 2nd ed., Columbia University Press, 305-8
DANGER
Hazard Statements H315 - H319 - H334 - H335
Precautions P261 - P280
Reaction P302 +P352 - P304 +P340 - P305 +P351 +P338 - P342 +P311

EG-Index: 647-008-00-6 • EINECS: 232-629-3 • WGK: 1 • HS: 35079090
Storage Temperature: -15 °C to -25 °C

Certificates of Analysis (Lot.-no. - release date)

Lot No.: 240102 — February 7, 2024
Lot No.: 231171 — November 30, 2023
Lot No.: 231136 — November 27, 2023
Lot No.: 230637 — June 7, 2023
Lot No.: 230455 — April 18, 2023
Lot No.: 221224 — January 11, 2023
Lot No.: 211346 — June 7, 2023
Lot No.: 211273 — November 4, 2021
Lot No.: 210821 — July 9, 2021
Lot No.: 210605 — May 18, 2021
Lot No.: 210397 — June 7, 2023
Lot No.: 210107 — February 3, 2021
Lot No.: 201058 — October 7, 2020
Lot No.: 201050 — October 6, 2020
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Lot No.: 200510 — May 15, 2020
Lot No.: 191259 — December 18, 2019
Lot No.: 190981 — March 31, 2021
Lot No.: 190755 — July 23, 2019
Lot No.: 190362 — April 11, 2019
Lot No.: 180968 — September 17, 2018
Lot No.: 180853 — April 11, 2019
Lot No.: 180069 — January 23, 2018
Lot No.: 170516 — February 11, 2019
Lot No.: 170095 — February 11, 2019
Lot No.: 160768 — August 15, 2016
Lot No.: 151383 — November 11, 2015
Lot No.: 150421 — April 9, 2015
Lot No.: 150317 — March 23, 2015
Lot No.: 141207 — November 11, 2015
Lot No.: 141023 — September 24, 2014
Lot No.: 140865 — July 7, 2015
Lot No.: 140571 — October 13, 2015
Lot No.: 140255 — February 24, 2014
Lot No.: 140176 — February 6, 2014
Lot No.: 140027 — January 14, 2014
Lot No.: 131136 — August 11, 2014
Lot No.: 130875 — August 12, 2013
Lot No.: 130362 — November 24, 2014
Lot No.: 130022 — January 15, 2013
Lot No.: 121039 — July 4, 2013
Lot No.: 120654 — June 5, 2012
Lot No.: 120294 — March 12, 2012
Lot No.: 111421 — July 3, 2012

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