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> Polymyxin B (Sulfate) [1405-20-5]

Polymyxin B (Sulfate) [1405-20-5]

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Cat# HY-A0248-5g

Size : 5g

Brand : MedChemExpress

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Description

Polymyxin B Sulfate is a potent antibacterial agent and a relatively toxic antibiotic. Polymyxin B Sulfate also is a antiendotoxin agent. Polymyxin B Sulfate shows endotoxin-neutralizing properties can be used as adjunctive research in gram-negative sepsis. Polymyxin B Sulfate shows antibacterial activities in vitro and in vivo[1][2][3].

In Vitro

Polymyxin B Sulfate shows antibacterial activities with MICs of 0.5 mg/l for E. coli strain IH3080[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Polymyxin B Sulfate Related Antibodies
In Vivo

Polymyxin B Sulfate (0.5-120 mg/kg; s.c.) shows antibacterial activities in thigh or lung infection mouse model[2].
Polymyxin B Sulfate (2 mg/kg, s.c.) shows potent in mouse bactericidal effect against E. coli strain IH3080[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Eight-week-old, 24-30 g, female Swiss mice[2]
Dosage: 0.5-120 mg/kg for thigh infection model; 5-120 mg/kg for lung infection mode
Administration: S.c.
Result: Showed antibacterial activities for three K. pneumoniae strains.
Animal Model: 7-9 weeks, female NMRI mice (E. coli IH3080)[3]
Dosage: 2 mg/kg
Administration: S.c.
Result: Decreased the bacterial count in a dose dependent manner.
Molecular Weight

1199.75 (average)

CAS No.
Appearance

Solid

Color

White to off-white

Structure Classification
Initial Source

Bacillus polymyxa
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 16.67 mg/mL (Need ultrasonic)

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 0.71 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 0.71 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.1 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 0.71 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 0.71 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (7.1 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

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