Application

• For quantitative determination of ammonia/ammonium and evaluation of drug effects on their metabolism.

Key Features

• Fast and sensitive. Linear detection range of 0.012 – 1 mM ammonia.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL360/450nm

Samples

• Biological (eg urine) and environ-mental samples

Species

• All

Procedure

• 15 min

Size

• 200 tests

Detection Limit

• 12 µM

Shelf Life

• 6 months

More Details

• AMMONIA (NH₃) or its ion form ammonium (NH₄⁺) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH₃ are very desirable. BioAssay Systems ammonia/ammonium assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 360/450nm) is proportional to the ammonia concentration in the sampl

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost