
Cross adsorbed Anti-Human secondary antibodies Anti-Human
Cross-adsorbed anti-Human antibodies represent a crucial tool in immunological research and diagnostics due to their exceptional specificity and versatility. These secondary antibodies are meticulously purified to eliminate any potential interference from antibodies recognizing shared epitopes on other proteins. This purification process ensures that the antibodies selectively target the desired human IgG, making them indispensable in a variety of applications.
Applications:
These antibodies find wide utility in a range of experimental techniques, including Western blotting, immunofluorescence, and immunohistochemistry. Their ability to detect and quantify human IgG with precision makes them invaluable for studying various biological processes, from protein expression profiling to disease biomarker analysis.
Forms and Conjugates:
Cross-adsorbed anti-Human antibodies are available in diverse forms to suit different experimental needs. They can be conjugated with various labels, such as Horseradish Peroxidase (HRP), Alexa Fluor, and CF Dyes, enabling researchers to choose the most appropriate detection method for their specific application.
Importance of Specificity:
In any experiment involving these antibodies, understanding the specificity of the polyclonal secondary antibody is paramount. This knowledge ensures accurate and reliable results, minimizing the risk of false positives or cross-reactivity.
Species-Specific and Multiplexing:
Cross-adsorbed secondary antibodies are designed to be species-specific, which means they primarily recognize human IgG, reducing the potential for background noise caused by non-specific binding. This specificity is particularly advantageous when conducting multiplex experiments, where multiple targets need to be simultaneously detected and distinguished with precision.
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- HRP 58
- Unconjugated 58
- AP 50
- FITC 50
- Biotin 47
- PE 21
- Rhodamine 11
- Texas Red® 10
- TRITC 9
- Alexa Fluor 488 8
- AMCA 7
- Alexa Fluor 647 7
- DyLight® 650 7
- DyLight® 550 6
- DyLight® 594 6
- Alexa Fluor 555 5
- DyLight® 488 5
- RPE 4
- Cy3 3
- Red 3
- Cy2 1
- Cy5 1
- DyLight® 405 1
- human 380
- goat 319
- rabbit 36
- donkey 24
- Secondary antibody
- ELISA 236
- FC 115
- WB 87
- IHC 82
- IF 77
- FLISA 40
- Microscopy 35
- Cellular activation 32
- ELISPOT 26
- IP 17
- Purification 11
- Cell depletion 9
- Multiplex assay 9
- ICC 2
- Dot blot 1
- Polyclonal 357
- polyclonal 3