Human monomers - Negative controls

T lymphocytes play a central role in immune system function.

Monomeric MHC/peptide complexes proved to be impractical due to their instability and low affinity yo TCRs. To overcome this difficulty, MHC/peptide monomers are biotinylated and tetramerized with streptavidin to maintain stable binding to multiple TCRs, enabling MHC/peptide tetramers to be used as detection tools.

Biotinylated MHC monomers, the building blocks of MHC tetramers, are now available for tetramer construction flexibility and novel applications.

The Biotinylated Class I MHC/peptide complex can be used in research studies involving CD8+ antigen-specific T cells. The Biotinylated Class II MHC/peptide complex can be used in research studies involving CD4+ antigen-specific T cells

Biotinylated MHC Class I monomer is composed of a particular peptide in combination with a particular HLA class I allele that has been biotinylated at the c-terminus of the heavy chain extracellular domain.

Biotinylated MHC class II monomer is composed of a particular peptide in combination with a particular HLA class II allele that has been biotinylated at the c-terminus of the beta chain extracellular domain.