The NeoPro Pico Western ECL Substrate, developed by Neo Biotech, is an advanced luminol-based enhanced chemiluminescent substrate designed to offer superior sensitivity and reliability for immunoblotting applications. Specifically formulated for use with horseradish peroxidase (HRP)-conjugated secondary antibodies, this substrate enables the detection of even the most low-abundant antigens, with a detection range spanning from mid-femtograms to high-femtograms. Its exceptional sensitivity ensures that even the faintest protein signals are captured with clarity, making it ideal for challenging samples where high sensitivity is crucial.

One of the key features of the NeoPro Pico is its extended signal duration, allowing for flexible imaging options. Whether using digital imaging systems or traditional film-based methods, the prolonged signal provides consistent results without the risk of signal degradation over time. To achieve optimal signal intensity and duration, it is recommended to fine-tune the dilutions of both primary and secondary antibodies. This level of customization ensures reliable, high-quality Western blot results across a variety of experimental conditions.