In the extracellular space, ecto-5′-nucleotidase (CD73) dephosphorylates adenosine triphosphate (ATP) to produce adenosine. Adenosine has four receptors namely A1R, A2AR A2BR and A3R. Adenosine plays a key role in the osteogenic differentiation. A1R induces osteoclast differentiation and A2AR induces osteoblast differentiation.||Adenosine has been used:|• as a supplement in embryonic type culture medium for in vitro osteogenic differentiation|• for evaluation of its effect on cell survival by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT assay) and cell proliferation by 3H-thymidine incorporation assay in multi drug resistant glioblastoma stem-like cells (GSCs), and as a standard in high performance liquid chromatography (HPLC).|• as a constituent in collagenase solution for the collagenase digestion of mice fat tissue.||Synonyms:|9-β-D-Ribofuranosyl-9H-purin-6-amine; 9-β-D-Ribofuranosyladenine; Adenine Riboside; Adenocard; Adenocor; Adenogesic; Adenoscan; Adrekar; Boniton; D-Adenosine; 1-(6-Amino-9H-purin-9-yl)-1-deoxy-β-D-ribofuranose; Myocol; NSC 7652||CAS No:|58-61-7||Molecular Formula:|C10H13N5O4||Molecular Weight:|267.25||Purity (HPLC):|≥99%||Appearance:|White to off-white powder||Melting Point:|230-234°C||Specific Optical Rotation:|-68.0º to -73º||Storage and Stability:|Store at 4°C. Stable for 12 months after receipt.