Our Anti-AKT (Thr342) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects drosophila AKT (Thr342) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of Drosophila lysate showing specific labeling of the ~68 kDa AKT protein phosphorylated at Thr342 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).