Anti-GST (GOAT) Antibody Biotin Conjugated
Cat# 600-106-200
Size : 1mg
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Specifications for GST Antibody Biotin Conjugated
Product Details
Anti-GST (GOAT) Antibody Biotin Conjugated - 600-106-200
goat anti-GST antibody biotin conjugation, biotin conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody
Goat
Biotin
Polyclonal
IgG
Target Details
GST-Tag
Native Protein
Glutathione-S-Transferase [Schistosoma japonicum]
Anti-GST Antibody, Biotin Conjugated, was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
Application Details
ELISA
IF - View References
Anti-GST Antibody, Biotin Conjugated has been tested by ELISA and is suitable for immunoblotting, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
Biotin conjugated anti-GST antibody is specific for the GST affinity tag. Affinity tags re appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag.
References (1)
Cao L et al. (2023). Regulation of branched versus linear Arp2/3-generated actin filaments. EMBO J.
Applications
IF, Confocal Microscopy PubMed
Related Protocols
ELISA Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Sandwich ELISA Protocol for Collagen
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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