Detection of human IgG2 in monkey serum. Sandwich ELISA using RM118 as the capture antibody, and biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by a HRP conjugated streptavidin. Sandwich ELISA using RM118 as the capture antibody, and biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. ELISA showing RM118 reacts only to human IgG2, and not to any other IgG subclasses (IgG1, IgG3, or IgG4), and no cross reactivity to IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. A titer ELISA using RM118. The plate was coated with different amounts of human IgG2. A serial dilution of RM118 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. ELISA showing RM118 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM118 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Product Details Product Name Anti-Human IgG2 Rabbit Monoclonal Antibody, Clone RM118 Product Description Rabbit monoclonal to Human IgG2 Catalog No. 31-1020-00 Clone Name RM118 Product Data Sheet Anti-Human IgG2 Rabbit Monoclonal Antibody Data Sheet RM118 Specificity RM118 reacts to the heavy chain of human IgG2. RM118 does not cross react to any other IgG subclasses (IgG1, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, or IgE. RM118 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. Immunogen Human IgG2 Application ELISA
Species Reactivity Human Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
References for Human IgG2 antibody [RM118] Sulka KB et al. Lyme Disease-Associated IgG4 Autoantibodies Correlate with Synovial Pathology in Antibiotic-Refractory Lyme Arthritis. Arthritis Rheumatol. (2018) 10.1002/art.40566. IHC-P; Human. Read more (PubMed: 29790305) . Product Images
Anti-Human IgG2 rabbit monoclonal antibody [RM118]
![Anti-Human IgG2 rabbit monoclonal antibody [RM118]](https://www.quimigen.pt/en/upload/-img-lapin1.png)
Cat# 31-1020-00
Size : 100ug
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Rabbit monoclonal to Human IgG2
100 µg
