| Product Details | |
| Product Name | Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119 |
| Product Description | Rabbit monoclonal to Human IgG3 |
| Catalog No. | 31-1021-00 |
| Clone Name | RM119 |
| Product Data Sheet | Anti-Human IgG3 Rabbit Monoclonal Antibody Data Sheet RM119 |
| Specificity | RM119 reacts to the heavy chain of human IgG3, RM119 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG4), and shows no cross reactivity IgM, IgA, IgD, IgE. RM119 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. |
| Immunogen | Human IgG3 |
| Application | ELISA Immunocytochemistry (ICC) Immunohistochemistry (IHC) |
| Species Reactivity | Human |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Properties | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 50 ng/well – 200 ng/well (for Capture); 0.05 µg/mL – 0.2 µg/mL (for Detection); Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL; Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL. |
| References for Human IgG3 antibody [RM119] | Sulka KB et al. Lyme Disease-Associated IgG4 Autoantibodies Correlate with Synovial Pathology in Antibiotic-Refractory Lyme Arthritis. Arthritis Rheumatol. (2018) 10.1002/art.40566. IHC-P; Human. Read more (PubMed: 29790305) |
| . | |
| Product Images | |
 | Detection of human IgG3 in monkey serum. Sandwich ELISA using RM119 as the capture antibody, and biotinylated anti-human light chains (κ+ λ) antibody, Clone RM129, as the detection antibody, followed by a HRP conjugated streptavidin. |
 | Sandwich ELISA using RM119 as the capture antibody (100 ng/well), and biotinylated anti-human light chains (κ+ λ) antibody, Clone RM129, as the detection antibody, followed by an HRP conjugated streptavidin. |
ELISA showing RM119 reacts only to human IgG3, and not to any other IgG subclasses (IgG1, IgG2, or IgG4), and no cross reactivity to IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. | |
 | A titer ELISA using RM119. The plate was coated with different amounts of human IgG3. A serial dilution of RM119 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human lymphoid tissue section using anti-human IgG3 rabbit monoclonal antibody, Clone RM119. |
 | ELISA showing RM119 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM119 and a monkey IgG binding antibody (positive control) was used as the detection antibody. |
 | Immunohistochemical staining of FFPE Tonsil tissue sections using Anti-Human IgG3 antibody, Clone RM119 at a 1:2000 dilution. Image courtesy of Shaomin Hu, MD, PhD, Department of Anatomic and Clinical Pathology. Montefiore Medical Center Albert Einstein College of Medicine. Bronx, New York, USA |
Anti-Human IgG3 rabbit monoclonal antibody [RM119]
![Anti-Human IgG3 rabbit monoclonal antibody [RM119]](https://www.quimigen.pt/en/upload/-img-lapin1.png)
Cat# 31-1021-00
Size : 100ug
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Rabbit monoclonal to Human IgG3
100 µg