RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3. Sandwich ELISA using RM217 as the capture antibody, and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. Sandwich ELISA using RM217 as the capture antibody, and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. A titer ELISA using RM217. The plate was coated with different amounts of human IgG4. A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Product Details Product Name Anti-Human IgG4 Fc Rabbit Monoclonal Antibody, Clone RM217 Product Description Rabbit monoclonal to Human IgG4 Fc Catalog No. 31-1100-00 Clone Name RM217 Product Data Sheet Anti-Human IgG4 Fc Rabbit Monoclonal Antibody Data Sheet RM217 Specificity RM217 reacts to the Fc region of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. Immunogen Peptide corresponding to the Fc region of Human IgG4 Application ELISA
Species Reactivity Human Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection). Keywords . Product Images
Anti-Human IgG4 Fc rabbit monoclonal antibody [RM217]
![Anti-Human IgG4 Fc rabbit monoclonal antibody [RM217]](https://www.quimigen.pt/en/upload/-img-lapin1.png)
Cat# 31-1100-00
Size : 100ug
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Rabbit monoclonal to Human IgG4 Fc
100 µg
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