Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-human Ig kappa light chain rabbit monoclonal antibody, Clone RM126. ELISA showing RM126 reacts only to the kappa light chain of human immunoglobulins, and not to lambda light chain, mouse IgG, rat IgG, or goat IgG. A titer ELISA using RM126. The plate was coated with different amounts of human IgG4κ. A serial dilution of RM126 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. ELISA showing RM126 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM126 and a monkey IgG binding antibody (positive control) was used as the detection antibody.
Product Details Product Name Anti-Human Kappa Light Chain Rabbit Monoclonal Antibody, Clone RM126 Product Description Rabbit monoclonal to Human Kappa Light Chain Catalog No. 31-1028-00 Clone Name RM126 Product Data Sheet Anti-Human Kappa Light Chain Rabbit Monoclonal Antibody Data Sheet RM126 Specificity RM126 reacts to the kappa light chain of human immunoglobulins, and does not react to the lambda light chain. RM126 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. Immunogen Human IgG Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA Species Reactivity Human Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 0.05 µg/mL – 0.2 µg/mL;
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL;
Flow Cytometry (FC): 0.5 µg/mL - 2 µg/mL. Keywords . Product Images
Anti-Human Kappa Light Chain rabbit monoclonal antibody [RM126]
Cat# 31-1028-00
Size : 100ug
Brand : RevMab Biosciences
Rabbit monoclonal to Human Ig kappa light chain
100 µg