ELISA of mouse immunoglobulins shows RM219 reacts to the Fc region of mouse IgG2a; no cross reactivity with IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. A titer ELISA of mouse IgG2a. The plate was coated with different amounts of mouse IgG2a. A serial dilution of RM219 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Western blot of nonreduced (-) and reduced (+) mouse IgG2a, using 0.5 µg/mL of RevMAb Clone RM219. This antibody reacts to nonreduced IgG2a (~150 kDa).
Product Details Product Name Anti-Mouse IgG2a Rabbit Monoclonal Antibody, Clone RM219 Product Description Rabbit monoclonal to Mouse IgG2a Catalog No. 31-1098-00 Clone Name RM219 Product Data Sheet Anti-Mouse IgG2a Rabbit Monoclonal Antibody Data Sheet RM219
Specificity RM219 reacts to the Fc region of mouse IgG2a. No cross reactivity with mouse IgG1, IgG2b, IgG2c, IgG3, IgM, IgA, IgE, human IgG, or rat IgG. Immunogen Mouse IgG2a Application ELISA
Western Blot (WB) (nonreduced only)
Species Reactivity Mouse Conjugate None Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 100 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 0.005 µg/mL – 0.2 µg/mL;
Western Blot (WB): 0.5 µg/mL - 2 µg/mL. Keywords . Product Images
Anti-Mouse IgG2a rabbit monoclonal antibody [RM219]
![Anti-Mouse IgG2a rabbit monoclonal antibody [RM219]](https://www.quimigen.pt/en/upload/-img-lapin1.png)
Cat# 31-1098-00
Size : 100ug
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Rabbit monoclonal to Mouse IgG2a
100 µg
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