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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-RABBIT IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)

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Cat# 611-101-122

Size : 1mg

Contact local distributor :

Phone : +1 850 650 7790

Brand : Rockland Immunochemicals

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Contact local distributor :

Phone : +1 850 650 7790

Specifications for Rabbit IgG (H&L) Antibody Pre-adsorbed

Product Details

Description: Goat Anti-Rabbit IgG (H&L) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) - 611-101-122

Synonyms: goat anti-Rabbit IgG Antibody, goat anti-Rabbit IgG Antibody pre-adsorbed, Rabbit Secondary Antibody

Host Species: Goat

Specificity: IgG (H&L)

Clonality: Polyclonal

Format: IgG

Target Details

Reactivity: Rabbit

Immunogen Type: Native Protein

Immunogen: Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.

Purity/Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. Specificity was confirmed using ELISA against at less than 1% of target signal.

Application Details

Tested Applications: ELISA, WB

Suggested Applications: Biochemical Assay, IF, LFA - View References

Application Note: Anti-Rabbit IgG (H&L) has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation

Concentration: 1.167 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Anti-Rabbit IgG (H&L) Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

References (2)

Geuijen C et al. (2021). A human CD137× PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade. Nat Commun.

Applications

IF, Confocal Microscopy

PubMed

Chen Z, Zhang Z, Zhai X, et al. (2020). Rapid and Sensitive Detection of anti-SARS-CoV-2 IgG, Using Lanthanide-Doped Nanoparticles-Based Lateral Flow Immunoassay. Anal Chem.

Applications

Lateral Flow (LFA)

PubMed

Related Protocols

Adherent Cell Lysis Protocol

Biotin Binding Assay Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Peptide Competition Assay (PCA) Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.