General Info
| Host: | Mouse |
| Applications: | WB/IF/FC |
| Reactivity: | Human/Mouse/Rat/Cow/Chicken/Pig/Sheep |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Mouse monoclonal antibody anti-Mothers against decapentaplegic homolog 5 (C-Term) is suitable for use in Western Blot, Immunofluorescence and Flow Cytometry research applications. |
| Clonality: | Monoclonal |
| Conjugation: | Unconjugated |
| Formulation: | Liquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol. |
| Purification: | Affinity purification |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000-1:2000IF 1:100-1:500FC 1:100-1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
| Gene Symbol: | SMAD5 |
| Gene ID: | 4090 |
| Uniprot ID: | SMAD5_HUMAN |
| Immunogen Region: | C-Term |
| Specificity: | Smad5 Monoclonal Antibody detects endogenous levels of Smad5 protein. |
| Immunogen: | Purified recombinant human Smad5 (C-terminus) protein fragments expressed in E.coli. |
Description
| Post Translational Modifications | Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. |
| Function | Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis. Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form an heteromeric complex which translocates into the nucleus acting as transcription factor. In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network. Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes. Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis. |
| Protein Name | Mothers Against Decapentaplegic Homolog 5Mad Homolog 5Mothers Against Dpp Homolog 5Jv5-1Smad Family Member 5Smad 5Smad5Hsmad5 |
| Database Links | Reactome: R-HSA-201451 |
| Cellular Localisation | CytoplasmNucleusMitochondrionCytoplasmic In The Absence Of LigandMigrates To The Nucleus When Complexed With Smad4 |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 5 antibodyAnti-Mad Homolog 5 antibodyAnti-Mothers Against Dpp Homolog 5 antibodyAnti-Jv5-1 antibodyAnti-Smad Family Member 5 antibodyAnti-Smad 5 antibodyAnti-Smad5 antibodyAnti-Hsmad5 antibodyAnti-SMAD5 antibodyAnti-MADH5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance