Antibody for the detection of FLAG™ conjugated proteins (RABBIT)
Cat# 600-401-383
Size : 250ug
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Specifications for DYKDDDDK Tag (Anti-FLAG®) Antibody
Product Details
Antibody for the detection of FLAG® conjugated proteins (RABBIT) - 600-401-383
rabbit antibody for the detection of FLAG™ conjugated proteins, rabbit anti DYKDDDDK
Rabbit
Polyclonal
IgG
Target Details
FLAG-Tag
Conjugated Peptide
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. This antibody reacts with FLAG™ conjugated proteins.
This affinity purified antibody is directed against the FLAG™ motif and is useful in determining its presence in various assays. This polyclonal anti-FLAG™ tag antibody detects over-expressed proteins containing the FLAG™ epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Application Details
ELISA, WB
IF, IHC, IP, Multiplex - View References
This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and FLAGä containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK. Rockland Immunochemical's polyclonal antibody to detect FLAG™ conjugated proteins binds FLAG™ containing fusion proteins with greater affinity than the widely used monoclonal M1, M2 and M5 clones, and shows greater sensitivity in most assays. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
Formulation
1.10 by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
References (58)
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Applications
WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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IF, Confocal Microscopy PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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IP, Co-IP PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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IF, Confocal Microscopy PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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IHC, ICC, Histology; WB, IB, PCA PubMed
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IHC, ICC, Histology; WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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Applications
IP, Co-IP PubMed
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WB, IB, PCA PubMed
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WB, IB, PCA PubMed
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Applications
IP, Co-IP; WB, IB, PCA PubMed
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Applications
Undefined PubMed
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Applications
WB, IB, PCA PubMed
Terra LF et al. (2019). Heat shock protein B1 is a key mediator of prolactin-induced beta-cell cytoprotection against oxidative stress. Free Radic Biol Med.
Applications
WB, IB, PCA PubMed
Olsen C et al. (2019). Regulation of somatostatin receptor 2 trafficking by C-tail motifs and the retromer. Endocrinology.
Applications
IF, Confocal Microscopy; WB, IB, PCA; Multiplex Assay PubMed
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Applications
WB, IB, PCA PubMed
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Undefined PubMed
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Applications
IP, Co-IP PubMed
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Applications
WB, IB, PCA PubMed
Shimo et al. (2018). Construction of a tri-chromatic reporter cell line for the rapid and simple screening of splice-switching oligonucleotides targeting DMD exon 51 using high content screening. PLOS One
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WB, IB, PCA PubMed
Yue et al. (2018). SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death. Cell Death & Disease
Applications
WB, IB, PCA PubMed
Smith et al. (2017). Protease-activated receptor-4 and P2Y12 Dimerize, Co-Internalize and Activate Akt Signaling via Endosomal Recruitment of β-arrestin. Journal of Biological Chemistry
Applications
IF, Confocal Microscopy; IP, Co-IP; WB, IB, PCA PubMed
Hu et al. (2017). Connexin 50 Functions as an Adhesive Molecule and Promotes Lens Cell Differentiation. Scientific Reports
Applications
WB, IB, PCA PubMed
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Applications
WB, IB, PCA PubMed
Grimsey et al. (2016). Recycling and Endosomal Sorting of Protease-activated Receptor-1 Is Distinctly Regulated by Rab11A and Rab11B Proteins. Journal of Biological Chemistry
Applications
E, EIA PubMed
Wang et al. (2016). S6K-STING interaction regulates cytosolic DNA-mediated activation of the transcription factor IRF3. Nature Immunology
Applications
WB, IB, PCA PubMed
Smith et al. (2016). Protease-activated Receptor-4 Signaling and Trafficking Is Regulated by the Clathrin Adaptor Protein Complex-2 Independent of β-Arrestins. Journal of Biological Chemistry
Applications
IF, Confocal Microscopy PubMed
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Applications
IP, Co-IP; WB, IB, PCA PubMed
Dores MR et al. (2015). GPCR sorting at multivesicular endosomes. Methods Cell Biol.
Applications
IF, Confocal Microscopy PubMed
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Applications
WB, IB, PCA PubMed
Ortiz-Sandoval CG. Et al. (2014). Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins. Cell Logist.
Applications
IP, Co-IP PubMed
Sinha S et al. (2014). Essential role of the chaperonin CCT in rod outer segment biogenesis. Invest Opthalmol. Vis Sci.
Applications
WB, IB, PCA PubMed
Liggett J et al. (2014). A novel COX-independent mechanism of sulindac sulfide involves cleavage of epithelial cell adhesion molecule protein. Exp Cell Res.
Applications
IF, Confocal Microscopy; Multiplex Assay PubMed
Dege C et al. (2014). Mi-2/NuRD chromatin remodeling complexes regulate B and T-lymphocyte development and function. Immunol Rev.
Applications
WB, IB, PCA PubMed
Gassen NC et al. (2014). Association of FKBP51 with priming of autophagy pathways and mediation of antidepressant treatment response: evidence in cells, mice, and humans. PLoS Med.
Applications
WB, IB, PCA PubMed
Gao X et al. (2013). Splice isoforms of phosducin-like protein control the expression of heterotrimeric G proteins. J Biol Chem.
Applications
WB, IB, PCA PubMed
Hara H et al. (2013). Phosphorylation of the adaptor ASC acts as a molecular switch that controls the formation of speck-like aggregates and inflammasome activity. Nat Immunol.
Applications
WB, IB, PCA PubMed
Badeaux AI et al. (2012). Loss of the methyl lysine effector protein PHF20 impacts the expression of genes regulated by the lysine acetyltransferase MOF. Biol Chem.
Applications
WB, IB, PCA PubMed
Raghavan B et al. (2012). Metal allergens nickel and cobalt facilitate TLR4 homodimerization independently of MD2. EMBO Rep.
Applications
WB, IB, PCA PubMed
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Applications
WB, IB, PCA PubMed
Albus CA et al. (2010). Y3IP1, a nucleus-encoded thylakoid protein, cooperates with the plastid-encoded Ycf3 protein in photosystem I assembly of tobacco and Arabidopsis. Plant Cell.
Applications
WB, IB, PCA PubMed
Related Protocols
Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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