Sandwich ELISA using RM117 as the capture Sandwich ELISA using RM117 as the capture ELISA of human immunoglobulins shows RM117 A titer ELISA using RM117. The plate was
Product Details Product Name Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody, Clone RM117 Product Description Biotinylated Rabbit monoclonal to Human IgG1 Catalog No. 31-1019-02 Clone Name RM117 Product Data Sheet Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody Data Sheet RM117 Specificity RM117 reacts to the heavy chain of human IgG1. No cross reactivity with human IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. Immunogen Peptide corresponding to the hinge region of Human IgG1 Application Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA Species Reactivity Human Conjugate Biotin Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 50 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL;
Flow Cytometry (FC): 2 µg/mL - 8 µg/mL. Keywords . Product Images
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
only reacted to human IgG1. No cross reactivity
with Human IgG2, IgG3, IgG4, IgE, IgD, IgA,
mouse IgG, rat IgG, or goat IgG. The plate was
coated with 50 ng/well of different immunoglobulins.
200 ng/mL, 50 ng/mL, or 10 ng/mL of RM117 was used
as the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary antibody.
coated with different amounts of human IgG1.
A serial dilution of RM117 was used as
the primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.
Biotin Anti-Human IgG1 rabbit monoclonal antibody [RM117]
![Biotin Anti-Human IgG1 rabbit monoclonal antibody [RM117]](https://www.quimigen.pt/en/upload/-img-lapin1.png)
Cat# 31-1019-02
Size : 50ug
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Biotinylated Rabbit monoclonal to Human IgG1
50 µg
