Sandwich ELISA using RM120 as the capture antibody, and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. Sandwich ELISA using RM120 as the capture antibody, and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG. A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. Immunohistochemistry of Human Lymphoid Tissue using Anti-Human IgG4 antibody, Clone RM120.
Product Details Product Name Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120 Product Description Biotinylated Rabbit monoclonal to Human IgG4 Catalog No. 31-1022-02 Clone Name RM120 Product Data Sheet Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody Data Sheet RM120
Specificity RM120 reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. Immunogen Peptide corresponding to the hinge region of Human IgG4 Application ELISA
Western Blot
Immunohistochemistry (IHC)
Immunocytochemistry (ICC)
Flow Cytometry (FC) Species Reactivity Human Conjugate Biotin Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 50 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Western Blot (WB): 0.5 µg/mL – 2 µg/mL;
Immunocytochemistry (ICC): 1 µg/mL - 10 µg/mL;
Immunohistochemistry (IHC): 1 µg/mL - 10 µg/mL;
Flow Cytometry (FC): 1 µg/mL - 4 µg/mL.
Keywords . Product Images
Biotin Anti-Human IgG4 rabbit monoclonal antibody [RM120]
![Biotin Anti-Human IgG4 rabbit monoclonal antibody [RM120]](https://www.quimigen.pt/en/upload/-img-lapin1.png)
Cat# 31-1022-02
Size : 50ug
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Biotinylated Rabbit monoclonal to Human IgG4
50 µg
