Western blot of nonreduced (-) and reduced (+) rabbit IgG (20 ng/lane), using 0.2 µg/mL of RevMAb Clone RMG02. This antibody reacts to nonreduced rabbit IgG (~150 kDa). ELISA of IgGs from different species shows RMG02 reacts to the Fc region of rabbit IgG; no cross reactivity with human IgG, rat IgG, or mouse IgG. The plate was coated with 50 ng/well of different IgG. 500 ng/mL, 200 ng/mL, or 50 ng/mL of RMG02 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody. A titer ELISA of rabbit IgG. The plate was coated with different amounts of rabbit IgG. A serial dilution of RMG02 was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
Product Details Product Name Biotin Anti-Rabbit IgG Fc Goat Monoclonal Antibody, Clone RMG02 Product Description Biotinylated Goat monoclonal to Rabbit IgG Fc
Catalog No. 41-1015-02 Clone Name RMG02
Product Data Sheet Biotin Anti-Rabbit IgG Fc Goat Monoclonal Antibody Data Sheet RMG02
Specificity RMG02 reacts to the Fc region of Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG. Immunogen Rabbit IgG
Application ELISA
Western Blot (WB) (nonreduced)
Species Reactivity Rabbit Conjugate Biotin Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 50 µg Concentration 1.0 mg/mL Purity Protein G affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Goat IgG
Usage ELISA: 0.01 µg/mL – 0.5 µg/mL;
Western Blot (WB) (nonreduced): 0.1 µg/mL - 0.5 µg/mL. Keywords . Product Images
Biotin Anti-Rabbit IgG Fc goat monoclonal antibody [RMG02]
Cat# 41-1015-02
Size : 50ug
Brand : RevMab Biosciences
Contact local distributor :
Phone : +1 850 650 7790
Biotinylated Goat monoclonal to Rabbit IgG Fc
50 µg
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