BRCC45 (BRE) (NM_199191) Human Tagged ORF Clone
Cat# RC220494
Size : 10µg
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BRCC45 (BRE) (NM_199191) Human Tagged ORF Clone
SKU
RC220494
BRE (Myc-DDK-tagged)-Human brain and reproductive organ-expressed (TNFRSF1A modulator) (BRE), transcript variant 3
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | BRCC45 |
| Synonyms | BRCC4; BRCC45; BRE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >RC220494 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCCAGAAGTGGCCTTGAACCGAATATCTCCAATGCTCTCCCCTTTCATATCTAGCGTGGTCCGGA ATGGAAAAGTGGGACTGGATGCTACAAACTGTTTGAGGATAACTGACTTAAAATCTGGCTGCACATCATT GACTCCTGGGCCCAACTGTGACCGATTTAAACTGCACATACCATATGCTGGAGAGACATTAAAGTGGGAT ATCATTTTCAATGCCCAATACCCAGAACTGCCTCCCGATTTTATCTTTGGAGAAGATGCTGAATTCCTGC CAGACCCCTCAGCTTTGCAGAATCTTGCCTCCTGGAATCCTTCAAATCCTGAATGTCTCTTACTTGTGGT GAAGGAACTTGTGCAACAATATCACCAATTCCAATGTAGCCGCCTCCGGGAGAGCTCCCGCCTCATGTTT GAATACCAGACATTACTGGAGGAGCCACAGTATGGAGAGAACATGGAAATTTATGCTGGGAAAAAAAACA ACTGGACTGGTGAATTTTCAGCTCGTTTCCTTTTGAAGCTGCCCGTAGATTTCAGCAATATCCCCACATA CCTTCTCAAGGATGTAAATGAAGACCCTGGAGAAGATGTGGCCCTCCTCTCTGTTAGTTTTGAGGACACT GAAGCCACCCAGGTGTACCCCAAGCTGTACTTGTCACCTCGAATTGAGCATGCACTTGGAGGCTCCTCAG CTCTTCATATCCCAGCTTTTCCAGGAGGAGGATGTCTCATTGATTACGTTCCTCAAGTATGCCACCTGCT CACCAACAAGGTGCAGTACGTGATTCAAGGGTATCACAAAAGAAGAGAGTATATTGCTGCTTTTCTCAGT CACTTTGGCACAGGTGTCGTGGAATATGATGCAGAAGGCTTTACAAAACTCACTCTGCTGCTGATGTGGA AAGATTTTTGTTTTCTTGTACACATTGACCTGCCTCTGTTTTTCCCTCGAGACCAGCCAACTCTCACATT TCAGTCCGTTTATCACTTTACCAACAGTGGACAGCTTTACTCCCAGGCCCAAAAAAATTATCCGTACAGC CCCAGATGGGATGGAAATGAAATGGCCAAAAGAGCAAAGGCTTATTTCAAAACCTTTGTCCCTCAGTTCC AGGAGGCAGCATTTGCCAATGGAAAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC220494 protein sequence Red=Cloning site Green=Tags(s) MSPEVALNRISPMLSPFISSVVRNGKVGLDATNCLRITDLKSGCTSLTPGPNCDRFKLHIPYAGETLKWD IIFNAQYPELPPDFIFGEDAEFLPDPSALQNLASWNPSNPECLLLVVKELVQQYHQFQCSRLRESSRLMF EYQTLLEEPQYGENMEIYAGKKNNWTGEFSARFLLKLPVDFSNIPTYLLKDVNEDPGEDVALLSVSFEDT EATQVYPKLYLSPRIEHALGGSSALHIPAFPGGGCLIDYVPQVCHLLTNKVQYVIQGYHKRREYIAAFLS HFGTGVVEYDAEGFTKLTLLLMWKDFCFLVHIDLPLFFPRDQPTLTFQSVYHFTNSGQLYSQAQKNYPYS PRWDGNEMAKRAKAYFKTFVPQFQEAAFANGKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms  Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map  |
| ACCN | NM_199191 |
| ORF Size | 1149 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_199191.3 |
| RefSeq Size | 1804 bp |
| RefSeq ORF | 1152 bp |
| Locus ID | 9577 |
| UniProt ID | Q9NXR7 |
| Cytogenetics | 2p23.2 |
| Protein Families | Druggable Genome |
| MW | 43.6 kDa |
| Summary | This gene encodes an anti-apoptotic, death receptor-associated protein that interacts with tumor necrosis factor-receptor-1. The encoded protein acts as an adapter in several protein complexes, including the BRCA1-A complex and the BRISC complex. The BRCA1-A complex possesses ubiquitinase activity and targets sites of double strand DNA breaks, while the BRISC complex exhibits deubiquitinase activity and is involved in mitotic spindle assembly. This gene is upregulated in several types of cancer. [provided by RefSeq, Jun 2016] |
| Product Manuals |
| FAQs |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC220494L3 | Lenti ORF clone of Human brain and reproductive organ-expressed (TNFRSF1A modulator) (BRE), transcript variant 3, Myc-DDK-tagged | 10 ug | | |
| RC220494L4 | Lenti ORF clone of Human brain and reproductive organ-expressed (TNFRSF1A modulator) (BRE), transcript variant 3, mGFP tagged | 10 ug | | |
| RG220494 | BRE (tGFP-tagged) - Human brain and reproductive organ-expressed (TNFRSF1A modulator) (BRE), transcript variant 3 | 10 ug | MSRP MSRP | |
| SC307897 | BRE (untagged)-Human brain and reproductive organ-expressed (TNFRSF1A modulator) (BRE), transcript variant 3 | 10 ug | | |
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