CD3 epsilon antibody [CD3-12]
Cat# GTX11089-100ug
Size : 100ug
Brand : GeneTex
Contact local distributor :
Phone : +1 850 650 7790
![Anti-CD3 epsilon antibody [CD3-12] used in IHC (Paraffin sections) (IHC-P). GTX11089](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-CD3 epsilon antibody [CD3-12] used in IHC (Paraffin sections) (IHC-P). GTX11089")
Host | Rat |
|---|---|
Clonality | Monoclonal |
Clone Name | CD3-12 |
Isotype | IgG1 |
Application | WB, ICC/IF, IHC-P, IHC-Fr, FACS |
Reactivity | Human, Mouse, Rabbit, Bovine, Cat, Dog, Chicken, Pig, Alpaca, Amazon Parrot, Bullfrog, Cynomolgus monkey, Duck, Great horned owl (Bubo virginianus), Harbour Porpoise, Horse, Koala, Raccoon, Rhesus Monkey, Sea Lion, Spotted Hyena, Xenopus |
| Package | 100 μg |
Datasheet File View product citations for antibody GTX11089 on CiteAb
PRODUCT
Summary
Rat monoclonal [CD3 - 12] to CD3
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| WB | Assay dependent |
| ICC/IF | Assay dependent |
| IHC-P | Assay dependent |
| IHC-Fr | Assay dependent |
| FACS | Assay dependent |
Not tested in other applications.
Calculated MW
23 kDa. ( Note )
Positive Control
Tonsil.
Product Note
This antibody has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC)
Purification
Protein G purified
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Unconjugated
RRID
AB_369097
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD3e molecule , IMD18 , T3E , TCRE
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Database
DATA IMAGES
| | GTX11089 IHC-P Image IHC-P analysis of human tonsil tissue using GTX11089 CD3 antibody [CD3-12]. |
| | GTX11089 WB ImageWB analysis of Jurkat cell lysate using GTX11089 CD3 antibody [CD3-12]. |
| | GTX11089 FACS Image FACS analysis of red cell lysed human peripheral blood gated on leukocytes using GTX11089 CD3 antibody [CD3-12]. |
Application Reference
Lago C et al. EMBO Mol Med 2023; 15 (12) : e18199 Patient- and xenograft-derived organoids recapitulate pediatric brain tumor features and patient treatments.
Clone Reference
Huiwen Zheng et al. Cell Rep 2022; 39 (8):110864 Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.
Nagatake T et al. Mucosal Immunol 2022; (Epub) Intestinal microbe-dependent ω3 lipid metabolite αKetoA prevents inflammatory diseases in mice and cynomolgus macaques.
Sugita J et al. Nat Commun 2021; 12 (1):1910 Cardiac macrophages prevent sudden death during heart stress.
Sikai Ling et al. Nat Biomed Eng 2021; (Epub) Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice.
Submit a Reference REVIEW
Application Reference
Lago C et al. EMBO Mol Med 2023; 15 (12):e18199 Patient- and xenograft-derived organoids recapitulate pediatric brain tumor features and patient treatments.
Fadi Jacob et al. bioRxiv 2020; (Epub) Generation and biobanking of patient-derived glioblastoma organoids and their application in CAR-T cell testing.
Clone Reference
Huiwen Zheng et al. Cell Rep 2022; 39 (8):110864 Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.
Nagatake T et al. Mucosal Immunol 2022; (Epub) Intestinal microbe-dependent ω3 lipid metabolite αKetoA prevents inflammatory diseases in mice and cynomolgus macaques.
Sugita J et al. Nat Commun 2021; 12 (1):1910 Cardiac macrophages prevent sudden death during heart stress.
Sikai Ling et al. Nat Biomed Eng 2021; (Epub) Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice.
Kirby L et al. Nat Commun 2019; 10 (1):3887 Oligodendrocyte precursor cells present antigen and are cytotoxic targets in inflammatory demyelination.
Zhang L et al. Mol Cell Probes 2018; 41:43-51 Immunopotentiating effect of Inonotus obliquus fermentation products administered at vaccination in chickens.
Maeda T et al. Int J Cancer 2018; (Epub) Immune-mediated antitumor effect of a transplanted lymph node.
Maeda T et al. Lymphat Res Biol 2017; (Epub) Lymphatic Reconnection and Restoration of Lymphatic Flow by Nonvascularized Lymph Node Transplantation: Real-Time Fluorescence Imaging Using * Green and Fluorescein Isothiocyanate-Dextran.
Ni G et al. BMC Immunology 2017; (Epub) Blocking IL-10 signalling at the time of immunization does not increase unwanted side effects in mice.
Sun F et al. PLoS One 2016; 11 (8):e0160659 Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine.
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