Choline/Acetylcholine Fluorometric Assay Kit
Cat# E-BC-F055-96T
Size : 96T
Brand : Elabscience
Contact local distributor :
Phone : +1 850 650 7790
| Detection Principle | Choline and acetylcholine play important roles in many biological processes. Choline is an essential nutrient, often grouped with B complex vitamins, that plays a key role in many biological processes. Choline is a precursor to the synthesis of acetylcholine (AchE), an important neurotransmitter in the peripheral and central nervous systems. Choline is measured by enzyme coupling reaction to generate fluorescent products in this kit, and the content of fluorescent substances is proportional to the presence of choline. Acetylcholinesterase was added to the reaction to hydrolyze acetylcholine into choline and acetic acid, and the acetylcholine content was further calculated by measuring the total amount of choline and acetylcholine. |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.06 μmol/L |
| Detection Range | 0.06-10 μmol/L |
| Precision | inter-assay CV: 8.2-10% | intra-assay CV: 3.2-5% |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse heart tissue homogenate | 10-20 |
| 10% Rat liver tissue homogenate | 10-20 |
| 10% Mouse lung tissue homogenate | 10-20 |
| 10% Mouse kidney tissue homogenate | 15-25 |
| 10% Mouse brain tissue homogenate | 15-25 |
| 10% Mouse liver tissue homogenate | 10-20 |
| Human serum | 3-5 |
| Rat serum | 3-7 |
| Rat plasma | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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Biotin
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Cyanine 5
Cyanine 5.5
Cyanine 7
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