Cholinesterase (CHE) Activity Assay Kit

Cat# E-BC-K052-S-100

Size : 100Assays

Brand : Elabscience

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Detection Principle Cholinesterase breaks down acetylcholine into choline and acetic acid. Acetylcholine that is not hydrolyzed by cholinesterase reacts with basic hydroxylamine to form acetamidamine. It reacts in an acidic solution to form a brown-red hydroxamate iron complex. The color depth is directly proportional to the amount of remaining acetylcholine, which can be colorimetrically quantified. Cholinesterase activity was calculated.
Synonyms CHE
Sample Type Whole blood,Serum,Plasma,Tissue,Cell
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (520 nm)
Research Area Liver And Renal Function
Other Reagents Required Normal saline (0.9% NaCl)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 1.17 U/mL
Detection Range 1.17-160 U/mL
Precision inter-assay CV: 9.4% | intra-assay CV: 3.7%
Assay Time 1 h
Cat.No. Product Name Sizes
E-BC-K125-S Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples) 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 2-3
Human plasma 2-3
Mouse serum 2-3
Mouse plasma 2-3
SH-SY5Y cells 1
10% Mouse brain tissue homogenate 1
10% Rat brain tissue homogenate 1
10% Rat kidney tissue homogenate 1
10% Rat spleen tissue homogenate 1
10% Mouse liver tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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