Assay Principle

The DetectX hours, offers a blend of efficiency and accuracy. Read the complete kit insert for comprehensive instructions before starting the assay. A cortisone standard is included to establish a precise standard curve.

Protocol Summary

• Add standards or diluted samples to the transparent microtiter plate provided. The plate is coated with goat anti-rabbit IgG antibody.
• Introduce cortisone peroxidase conjugate and cortisone polyclonal rabbit antibody to begin the immunological reaction.
• Incubate the mixture for 2.5 hours at room temperature with shaking, noting that the reaction inversely correlates with the cortisone concentration in the sample.
• After incubation, wash away excess conjugate and add the TMB substrate, which reacts with the bound conjugate to produce a measurable signal.
• Use a plate reader to detect the signal at 450nm and calculate cortisone concentration using the standard curve.

Background

Cortisone (C21H28O5), also known as Kendallβ-hydroxysteroid dehydrogenase (11β-HSD) enzymes regulate the balance between cortisol and cortisone. 11β-HSD1, mainly found in the liver, converts cortisone to cortisol, whereas 11β-HSD2, present in kidney tissues, is vital for cortisol receptor binding.

This glucocorticoid shuttle is crucial in initiating and regulating anti-inflammatory responses in the body. The dynamic interplay between cortisol and cortisone is pivotal in research on stress physiology, endocrine disorders, and inflammatory conditions. Furthermore, cortisone id="related-products-heading">Related News