OKAU00002-2PLATE | Creatinine Urinary Detection Kit Quimigen Portugal

Technical Documents

Datasheets/Manuals	Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.

Product Info

Application

ELISA-Sandwich

ELISA Kit Linearity

Linearity was determined by taking two diluted 1:20 human urine samples, one with a low diluted creatinine level of 0.38 mg/dL and one with a higher diluted level of 9.33 mg/dL and mixing them in the ratios given below. The measured concentrations were compared to the expected values.

High Urine	Low Urine	Observed Conc. (mg/dL)	Expected Conc. (mg/dL)	% Recovery
80%	20%	7.75	7.54	102.8
60%	40%	5.89	5.75	102.4
40%	60%	4.12	3.96	104.0
20%	80%	2.29	2.17	105.5
			Mean Recovery	103.7%

ELISA Kit Principle

The DetectXnm wavelength. The concentration of the creatine in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers. The Jaffe reaction used in this kit has been modified to read creatinine levels in urine.

ELISA Kit Reproducibility

Intra Assay Precision Four human urine samples were diluted 1:20 with deionized water and run in replicates of 20 in an assay. The mean and precision of the calculated creatinine concentrations were:

Sample	Creatinine Conc. (mg/dL)	%CV
1	8.92	2.8
2	4.08	1.3
3	1.94	2.5
4	1.11	3.0

Inter Assay Precision Four human urine samples were diluted 1:20 with deionized water and run in duplicates in 20 assays run over five days by three operators. The mean and precision of the calculated creatinine concentrations were:

Sample	Creatinine Conc. (mg/dL)	%CV
1	9.04	2.3
2	4.18	2.7
3	2.03	3.9
4	1.18	3.7

ELISA Kit Component

Component	Quantity
Clear 96 Well Plates	2 plates,2 by 5 plates
Creatinine Standard	1 mL,2 by 1 mL
DetectX mL,2 by 50 mL
Plate Sealers	2 each,10 each

Additional Information

Background: Creatinine (2-amino-1-methyl-5H-imidazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine. Under normal conditions, its formation occurs at a rate that is relatively constant and as intra-individual variation is < 15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Additionally, altered creatinine levels may be associated with other conditions that result in decreased renal blood flow such as diabetes and cardiovascular disease.

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Detection Limit: 0.037 mg/dL

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Cross Reactivity: It is well known that some typical components of human urine may interfere with the Jaffe reaction for creatinine measurement in urine. A diluted urine sample was spiked with 2,000 mg/dL of glucose (equivalent to 40,000 mg/dL undiluted) and tested in the kit. The unspiked diluted sample read at 8.44 mg/dL. No significant change to the measured creatinine level was seen at any glucose concentration.

Reconstitution and Storage

29 mg/dL

Target Info

Gene Full Name	Creatinine Urinary

Protocols & Data

Creatinine Urinary Detection Kit

Cat# OKAU00002-2PLATE

Contact local distributor :

Brand : Aviva Systems Biology

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Creatinine Urinary Detection Kit (OKAU00002)