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Measurement of 2DG uptake in differentiated 3T3-L1 adipocytes and 3T3-L1 fibroblasts.
Measurement of 2DG uptake in differentiated 3T3-L1 adipocytes and 3T3-L1 fibroblasts.
Assays were performed with Glucose Uptake Assay Kit (Cat # KA4086) in a black wall/clear bottom cell culture Poly-D lysine plate using a SpectraMax (Molecular Devices) microplate reader. (A: Negative Control, no insulin no 2-DG treatment. B: 2DG uptake in the absence of insulin. C: 2DG uptake in the presence of 1 mM insulin. D: 2DG uptake in the presence of 1 mM insulin and 200 mM phloretin. E: 2DG uptake in the presence of insulin 1 mM and 5 mM D-Glucose.) (Please refer to the protocol for detailed operations.)
- Specifications
Product Description
Glucose Uptake Assay Kit is used for the measurement of glucose uptake.
Suitable Sample
Live Cells
Absorbance (nm)
OD ratio at 570/610 nm
Detection Method
Colorimetric
Instrument Platform
Microplate Reader
Regulation Status
For research use only (RUO)
Storage Instruction
Store the kit in desiccated environment at -20°C and avoid from light.
Note
Measurement of 2DG uptake in differentiated 3T3-L1 adipocytes and 3T3-L1 fibroblasts.
- Applications
Quantification
- Publication Reference
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