Guinea Pig Polyclonal Anti-Keratin K14
Cat# GP-CK14
Size : 100µL
Brand : Progen
Contact local distributor :
Phone : +1 850 650 7790
| Quantity | 100 µl |
|---|---|
| Antibody Type | Polyclonal |
| Host | Guinea pig |
| Conjugate | Unconjugated |
| Application | IHC, WB |
| Purification | Stabilized antiserum |
| Reactivity | Human, Mouse |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Immunogen | Peptide against human K14 C-VSTHEQVLRTKN (460-472) |
| Formulation | Contains 0.09% sodium azide and 0.5% BSA |
| UniprotID | P02533 (Human), Q61781 (Mouse) |
| Synonym | Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14, KRT14 |
| Note | Centrifuge prior to opening |
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:100-1:200 |
| Immunohistochemistry (IHC) - paraffin | 1:50 (microwave treatment recommended) |
| Western Blot (WB) | 1:5,000-1:10,000 |
Reactive Polypeptide: acidic keratin K14 (Mr 50,000; formerly also designated cytokeratin 14), expressed in basal and first suprabasal layers of epidermis.
Tested cultured cell lines: BPH-1 (derived from non-neoplastic prostatic tissue).
Tumors specifically detected: the antiserum reacts with keratin K14, expressed in the basal cells of the larynx, esophagus, trachea, bladder, prostate, cervix, vagina, breast acini, skin and sweat glands. In several studies the correlation between the expression of keratin K14 in different types of carcinomas (putatively derived from basal epithelial cells) and prognosis has been discussed (see references).
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