H520 cells
Cat# C0016010
Size : OneFrozenvial
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Disease:
Squamous cell carcinoma
Origin:
This cell line was derived from a sample of a lung mass taken from a patient with squamous cell carcinoma of the lung
Species:
Homo sapiens Lung Epithelial Adherent This is a hypotriploid human cell line. The modal chromosome number is 58 occurring at 30%. Male AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:2 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C. Complete culture medium supplemented with 5% (v/v) DMSO I Bacteria: Negative Yeast: Negative Mycoplasma: Negative HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Amelogenin: X CSF1PO: 10 D13S317: 10,11 D16S539: 8,13 D5S818: 12,13 D7S820: 8,12 THO1: 10 TPOX: 8 vWA: 18,19 If use of this culture results in a scientific publication, it should be cited in the publication as: H520 (AddexBio C0016010) A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells Tissue:
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References for H520 cells:
1. Banks-Schlegel SP, Gazdar AF, Harris CC. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 1985 Mar;45(3):1187-1197. PMID: 2578876