Reagents and instruments for immunology, cell biology and molecular biology.
 
> Hoechst 33258

Hoechst 33258

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Cat# 40045

Size : 100MG

Brand : Biotium

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Hoechst

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains.

Hoechst 33342, 10 mg/mL in H2O
Hoechst 33258, 10 mg/mL in H2O
Hoechst 33258, Pentahydrate
Hoechst - Image 4

Product Attributes

CAS number

23491-45-4, 23491-52-3
Detection method/readout

Fluorescence microscopy, Flow cytometry
Probe cellular localization

Nucleus
For live or fixed cells

For fixed cells, For live/intact cells
Assay type/options

DNA content/cell cycle profiling by flow cytometry, Long term staining (24-72h), No-wash staining, Real-time imaging, Tissue staining
Cell permeability

Membrane permeant
Apoptosis/viability marker

All cell stain
Fixation options

Fix before staining (formaldehyde), Fix after staining (formaldehyde), Fix before staining (methanol), Fix after staining (methanol), Permeabilize after staining
Colors

Blue
Excitation/Emission

352/458 nm (with DNA)

Technology

Cellular Stains
Considerations for Immunofluorescence Staining

Tech Tips

Hoechst & DAPI Staining Protocols – Cell Staining with Hoechst or DAPI Nuclear Stains
Size
Catalog #
Qty
10 mL (10 mg/mL)
100 mg
40044

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