Human Cathepsin D antibody
Cat# ACH0627-50
Size : 50ul
Brand : NKMAX BIO
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Phone : +1 850 650 7790
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: Hep3B cell lysate
Lane 3.: MDA-MB-231 cell lysate
Lane 4.: MCF7 cell lysate
Lane 1.: HepG2 cell lysate
Lane 2.: Hep3B cell lysate
Lane 3.: MDA-MB-231 cell lysate
Lane 4.: MCF7 cell lysate
The recombinant proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Recombinant Human CTSB
Lane 2. : Recombinant Human CTSD
Lane 3. : Recombinant Human CTSE
Lane 4. : Recombinant Human CTSF
Lane 5. : Recombinant Human CTSH
Lane 6. : Recombinant Human CTSK
Lane 7. : Recombinant Human CTSL
Lane 8. : Recombinant Human CTSS
Lane 9. : Recombinant Human CTSW
Lane 10. : Recombinant Human CTSZ
Lane 1. : Recombinant Human CTSB
Lane 2. : Recombinant Human CTSD
Lane 3. : Recombinant Human CTSE
Lane 4. : Recombinant Human CTSF
Lane 5. : Recombinant Human CTSH
Lane 6. : Recombinant Human CTSK
Lane 7. : Recombinant Human CTSL
Lane 8. : Recombinant Human CTSS
Lane 9. : Recombinant Human CTSW
Lane 10. : Recombinant Human CTSZ
Immunohistochemistry (IHC)
Paraffin embedded sections of human breast lobule tissue were incubated with anti-human Cathepsin D (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.