Human EphA2 antibody
Cat# AEA0909-50
Size : 50ul
Brand : NKMAX BIO
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The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EphA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: EphA2 extracellular domain transfected 293T cell lysate
Lane 3.: EphA2 cytoplasmic domain transfected 293T cell lysate
Lane 1.: 293T cell lysate
Lane 2.: EphA2 extracellular domain transfected 293T cell lysate
Lane 3.: EphA2 cytoplasmic domain transfected 293T cell lysate
Immunohistochemistry (IHC)
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.