Human IL-1β ELISA Kit

Interleukin-1β (IL-1β) is a pro-inflammatory cytokine primarily secreted by activated macrophages, monocytes, and  the slanDC dendritic cell subset in the form of a protein precursor. It becomes an active form through hydrolysis by  caspase 1 (CASP1/ICE). IL-1β plays a regulatory role in cell immune activation and the inflammatory response. It  stimulates CD4+ cells to differentiate into Th17 cells. When combined with IL-23, IL-1β induces γδ T cells to express  IL-17, IL-21, and IL-22. Together with IL-1α and IL-18, IL-1β coordinates immune responses through various  downstream mechanisms. IL-1β is involved in the regulation of IL-6 and TNF-α, activating the adhesion molecule  ICAM1. IL-1β participates in the body's hematopoietic system, nervous system, endocrine system, inflammatory  responses, and certain anti-tumour physiological processes, including sepsis, acute and chronic myeloid leukaemia,  atherosclerosis, type II diabetes, rheumatoid arthritis, osteoarthritis, inflammatory bowel disease, multiple sclerosis,  Crohn's disease, Alzheimer's disease, melanoma, colon cancer, lung cancer, breast cancer, etc. 

This assay kit employs enzyme-linked immunosorbent assay (ELISA) method with dual antibodies to quantitatively  determine the concentration of IL-1β in human serum, plasma, and cell culture supernatant. The kit utilizes a  high-affinity anti-human IL-1β antibody pre-coated on the enzyme-labelled plate. Standard samples or test samples  are added to the microplate wells, and after incubation, IL-1β present in the samples specifically binds to the  pre-coated antibody on the plate. After washing to remove unbound substances, biotin-labelled anti-human IL-1β  detection antibody is added to the microplate wells. Following another incubation, the detection antibody binds  specifically to IL-1β anchored on the enzyme-labelled plate. Subsequently, streptavidin-horseradish peroxidase  (Streptavidin-HRP) is added to the microplate wells and incubated. The high-intensity non-covalent binding between  biotin on the antibody and streptavidin forms a "pre-coated antibody - human IL-1β protein - detection antibody -  Streptavidin-HRP" immunocomplex. After another wash, the chromogenic substrate TMB is added to the microplate  wells, and HRP catalyses the generation of a blue product. The intensity of the chromogenic reaction is directly  proportional to the concentration of IL-1β in the sample. The reaction is terminated by adding a stop solution, and the  absorbance is measured at 450 nm wavelength (reference wavelength 570 - 630 nm). By plotting a standard curve, the  concentration of IL-1β in the sample can be calculated from the absorbance values. This assay kit is highly specific,  sensitive, and convenient to operate.

Storage

at 2~8°C in the dark for one year

Shipping

ice bag (4℃)