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> Human S100 calcium binding protein B (S-100B) ELISA Kit

Human S100 calcium binding protein B (S-100B) ELISA Kit

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Cat# KTE60758-96

Size : 96T

Brand : Abbkine

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Phone : +1 850 650 7790

Specification

Product name Human S100 calcium binding protein B (S-100B) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human S100 calcium binding protein B (S-100B) ELISA Kit employs a two-site sandwich ELISA to quantitate S-100B in samples. An antibody specific for S-100B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS-100B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S-100B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S-100B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative S-100B; NEF; S100; S100beta; OTTHUMP00000174958; S-100 calcium-binding protein; beta chain; S100 beta; S100 calcium binding protein; beta (neural); S100 calcium-binding protein; beta; S100 calcium-binding protei

Product Properties

Kit components calcium binding protein B microplate
calcium binding protein B standard
calcium binding protein B detect antibody
calcium binding protein B (S-100B) ELISA Kit has high sensitivity and excellent specificity for detection of Human S-100B. No significant cross-reactivity or interference between Human S-100B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes minutes before opening.
minutes.
- 8 class="pro-form-title">Additional Information
Background S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B is glial-specific and is expressed primarily by astrocytes, but not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein.
Gene ID 6285
Alternative S-100B; NEF; S100; S100beta; OTTHUMP00000174958; S-100 calcium-binding protein; beta chain; S100 beta; S100 calcium binding protein; beta (neural); S100 calcium-binding protein; beta; S100 calcium-binding protei
Accession P04271

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