Hydroxyproline (HYP) Colorimetric Assay Kit (Acid Hydrolysis Method)

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Cat# E-BC-K062-M-96T

Size : 96T

Contact local distributor :

Phone : +1 850 650 7790

Brand : Elabscience

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Contact local distributor :

Phone : +1 850 650 7790

Detection Principle	The sample is hydrolyzed to generate free HYP, and hydroxyproline can produce oxidation product under the action of oxidizing agent. The generated oxidation product can react with chromogenic agent to produce burgundy. The concentration of hydroxyproline can be calculated by measuring the OD value at 558 nm.
Synonyms	HYP
Sample Type	Serum,Plasma,Animal tissue,Urine
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (550-570 nm, optimum wavelength: 558 nm)
Research Area	Amino Acids And Proteins
Other Reagents Required	6 mol/L Hydrochloric acid, Concentrated hydrochloric acid (12 mol/L), N-propyl alcohol
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.04 μg/mL
Detection Range	0.04-10 μg/mL
Precision	inter-assay CV: 4.4% \| intra-assay CV: 1.2%
Assay Time	50 min

Cat.No.	Product Name	Sizes
E-BC-K055-M	Total Amino Acids (T-AA) Colorimetric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Rat liver tissue homogenate	1
10% Rat kidney tissue homogenate	1
10% Rat lung tissue homogenate	1
10% Rat brain tissue homogenate	1
Chicken Tendon	20-30
Fish scale	20-30
Porcine cartilage	15-25
Human Urine	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.