IgG ELISA Kit (Sheep)
Cat# OKIA00186
Size : 1x96wellplate
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
IgG ELISA Kit (Sheep) (OKIA00186)
| Datasheets/Manuals | Printable datasheet for IgG ELISA Kit (Sheep) (OKIA00186) |
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| Predicted Species Reactivity | Sheep | ||||||||||||||||
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| Application | ELISA | ||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||
| ELISA Kit Duration | 50 Minutes | ||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology IgG ELISA Kit (Sheep) (OKIA00186) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for IgG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-IgG - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IgG captured in the well. | ||||||||||||||||
| ELISA Kit Range | 15.625 ng/ml - 500 ng/ml | ||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Intended Use: The total Sheep IgG test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG in sheep biological samples. | ||||||||||||||||
| :: | REAGENTS 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 20X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 L of affinity purified anti-Sheep IgG antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-SHEEP IgG ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Sheep IgG. 7. SHEEP IgG CALIBRATOR One vial containing Sheep IgG calibrator. | ||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Sensitivity | 4.280 ng/ml | ||||||||||||||||
| Predicted Homology Based on Immunogen Sequence | Sheep | ||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Alias Symbols | IGHG1, Ig gamma, Immunoglobulin G, Ig gamma-1 chain C region |
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| Protein Name | IgG |