Importin 8 (IPO8) (NM_006390) Human Tagged ORF Clone
Cat# RC215167
Size : 10ug
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| Product Data | |
| Target Symbol | Importin 8 |
|---|---|
| Synonyms | RANBP8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | ORF Nucleotide Sequence >RC215167 representing NM_006390 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTCAACCGGATCATCCAGGCGCTGAAGGGCACCATCGACCCGAAGTTGCGGATTGCAGCCGAGA ACGAGCTCAACCAGTCCTACAAGATTATCAATTTTGCCCCCAGTTTACTTCGGATTATAGTCTCTGACCA TGTGGAATTCCCAGTACGACAGGCAGCTGCCATTTACCTGAAGAACATGGTGACACAATACTGGCCAGAT CGAGAACCTCCACCAGGAGAAGCAATATTTCCATTCAACATTCACGAAAACGATCGCCAGCAAATACGTG ATAACATTGTGGAAGGAATAATTCGGTCTCCAGATTTAGTGAGAGTCCAATTAACAATGTGTCTCCGTGC CATCATAAAACATGATTTTCCTGGTCACTGGCCAGGAGTGGTCGACAAGATAGACTATTACTTGCAATCA CAGAGCAGTGCAAGCTGGCTTGGCAGTTTATTATGCCTGTATCAACTGGTGAAGACATATGAATATAAGA AAGCAGAAGAGAGAGAACCTCTTATAATAGCAATGCAGATATTCCTGCCTCGTATTCAGCAACAAATTGT TCAGCTCCTTCCTGATTCCTCCTATTATTCTGTATTACTGCAGAAACAAATTCTGAAAATCTTTTATGCA CTTGTTCAGTATGCATTGCCTCTTCAGCTAGTGAATAACCAAACCATGACAACATGGATGGAGATCTTCC GAACTATTATCGACAGGACCGTTCCTCCTGAGACTCTGCACATTGATGAGGATGATAGACCAGAACTGGT ATGGTGGAAGTGTAAGAAGTGGGCACTGCATATTGTAGCTCGGCTCTTTGAACGATATGGAAGCCCAGGA AATGTCACAAAAGAATACTTTGAATTTTCTGAATTCTTTTTGAAAACCTATGCAGTGGGCATTCAGCAGG TGCTACTAAAAATTTTAGATCAATATAGACAGAAAGAATATGTAGCTCCCCGTGTTCTTCAGCAAGCATT CAACTATCTCAACCAAGGGGTGGTTCATTCTATAACCTGGAAGCAGATGAAGCCACACATACAGAATATC TCTGAAGATGTGATTTTTTCTGTGATGTGTTATAAAGATGAGGATGAAGAGCTGTGGCAAGAAGATCCAT ATGAGTATATAAGGATGAAATTTGATATTTTTGAAGATTATGCTTCTCCCACCACAGCAGCCCAGACTCT CTTATATACTGCTGCAAAGAAAAGAAAAGAGGTGTTGCCAAAAATGATGGCATTCTGTTATCAAATCCTG ACAGACCCGAACTTTGACCCTAGGAAGAAAGATGGAGCCCTGCATGTGATTGGTTCCCTAGCTGAGATTT TACTGAAGAAGAGTTTATTCAAGGACCAAATGGAGCTGTTTCTACAAAATCATGTATTTCCATTATTATT GTCTAACCTGGGATATCTTCGAGCTAGATCTTGCTGGGTACTTCATGCATTTAGTTCTTTGAAGTTCCAT AATGAGCTCAATCTAAGAAATGCCGTTGAATTAGCGAAGAAGAGCCTGATTGAAGATAAAGAGATGCCTG TCAAAGTTGAAGCTGCCCTTGCTCTTCAGTCTTTAATTTCTAACCAGATACAAGCTAAGGAATATATGAA GCCACATGTGAGGCCTATTATGCAGGAACTGTTGCACATTGTTAGAGAGACAGAAAATGATGATGTTACT AATGTCATCCAGAAGATGATATGTGAATACAGTCAAGAGGTAGCCTCAATTGCTGTTGATATGACCCAAC ACTTGGCTGAGATATTTGGCAAAGTTCTTCAAAGTGATGAATATGAAGAAGTTGAAGACAAAACAGTAAT GGCTATGGGAATTTTACATACCATTGATACTATCTTAACAGTTGTAGAAGATCATAAAGAGATTACCCAG CAGTTAGAGAATATCTGTCTACGGATCATTGATCTTGTTCTGCAGAAACATGTAATTGAATTCTATGAAG AAATTCTTTCCCTGGCATACAGTTTAACCTGCCACAGTATTTCCCCTCAAATGTGGCAGCTTCTAGGTAT ACTATATGAAGTGTTTCAGCAGGATTGCTTTGAATACTTTACAGACATGATGCCTCTCCTGCATAATTAT GTGACAATAGATACAGATACCTTACTATCAAATGCAAAACATTTAGAAATTCTTTTTACAATGTGTAGGA AGGTACTATGTGGAGATGCAGGAGAAGATGCAGAGTGTCATGCAGCTAAACTTCTGGAAGTCATCATTCT TCAGTGCAAAGGAAGGGGAATTGATCAGTGCATTCCACTCTTCGTTCAACTTGTTTTGGAGAGATTAACT CGAGGGGTCAAAACTAGTGAGCTTCGTACTATGTGTCTTCAGGTTGCAATTGCTGCCTTGTACTACAACC CTGATTTGCTGCTACATACTTTAGAACGAATTCAGTTGCCTCACAACCCTGGACCTATCACTGTACAGTT TATAAATCAATGGATGAATGATACAGATTGTTTTCTTGGGCATCATGACCGGAAGATGTGTATAATAGGA CTGAGTATCCTTTTGGAATTGCAAAATCGACCTCCTGCAGTAGATGCTGTGGTGGGACAGATTGTTCCCT CAATTCTTTTCCTTTTCCTTGGCCTAAAGCAGGTCTGTGCTACTAGACAACTGGTAAACCGGGAAGATCG TTCAAAAGCAGAGAAAGCTGATATGGAAGAAAATGAGGAGATTTCAAGTGATGAAGAGGAGACAAATGTA ACTGCTCAAGCAATGCAGTCAAATAATGGAAGAGGTGAAGATGAGGAGGAGGAAGATGATGACTGGGATG AAGAAGTATTGGAAGAAACCGCGCTTGAGGGGTTCAGTACTCCACTTGACCTTGACAATAGTGTGGATGA ATATCAGTTTTTTACACAAGCTCTGATAACTGTGCAGAGTCGAGATGCAGCCTGGTACCAGCTGCTGATG GCACCACTCAGCGAGGATCAGAGGACAGCACTGCAGGAGGTGTACACACTGGCAGAGCACCGACGGACGG TGGCAGAGGCAAAGAAGAAGATTGAACAACAGGGAGGCTTCACCTTTGAAAACAAAGGAGTCCTCTCCGC ATTTAATTTTGGGACTGTGCCCAGCAACAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence >RC215167 representing NM_006390 Red=Cloning site Green=Tags(s) MDLNRIIQALKGTIDPKLRIAAENELNQSYKIINFAPSLLRIIVSDHVEFPVRQAAAIYLKNMVTQYWPD REPPPGEAIFPFNIHENDRQQIRDNIVEGIIRSPDLVRVQLTMCLRAIIKHDFPGHWPGVVDKIDYYLQS QSSASWLGSLLCLYQLVKTYEYKKAEEREPLIIAMQIFLPRIQQQIVQLLPDSSYYSVLLQKQILKIFYA LVQYALPLQLVNNQTMTTWMEIFRTIIDRTVPPETLHIDEDDRPELVWWKCKKWALHIVARLFERYGSPG NVTKEYFEFSEFFLKTYAVGIQQVLLKILDQYRQKEYVAPRVLQQAFNYLNQGVVHSITWKQMKPHIQNI SEDVIFSVMCYKDEDEELWQEDPYEYIRMKFDIFEDYASPTTAAQTLLYTAAKKRKEVLPKMMAFCYQIL TDPNFDPRKKDGALHVIGSLAEILLKKSLFKDQMELFLQNHVFPLLLSNLGYLRARSCWVLHAFSSLKFH NELNLRNAVELAKKSLIEDKEMPVKVEAALALQSLISNQIQAKEYMKPHVRPIMQELLHIVRETENDDVT NVIQKMICEYSQEVASIAVDMTQHLAEIFGKVLQSDEYEEVEDKTVMAMGILHTIDTILTVVEDHKEITQ QLENICLRIIDLVLQKHVIEFYEEILSLAYSLTCHSISPQMWQLLGILYEVFQQDCFEYFTDMMPLLHNY VTIDTDTLLSNAKHLEILFTMCRKVLCGDAGEDAECHAAKLLEVIILQCKGRGIDQCIPLFVQLVLERLT RGVKTSELRTMCLQVAIAALYYNPDLLLHTLERIQLPHNPGPITVQFINQWMNDTDCFLGHHDRKMCIIG LSILLELQNRPPAVDAVVGQIVPSILFLFLGLKQVCATRQLVNREDRSKAEKADMEENEEISSDEEETNV TAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALITVQSRDAAWYQLLM APLSEDQRTALQEVYTLAEHRRTVAEAKKKIEQQGGFTFENKGVLSAFNFGTVPSNN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms | Chromatograms Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_006390 |
| ORF Size | 3111 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@quimigen.pt The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006390.4 |
| RefSeq Size | 5178 bp |
| RefSeq ORF | 3114 bp |
| Locus ID | 10526 |
| UniProt ID | O15397 |
| Cytogenetics | 12p11.21 |
| Domains | IBN_NT |
| MW | 119.8 kDa |
| Summary | The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. This protein binds to the nuclear pore complex and, along with RanGTP and RANBP1, inhibits the GAP stimulation of the Ran GTPase. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
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| SDS |
| SKU | Description | Size | ||
|---|---|---|---|---|
| RC215167L3 | Lenti ORF clone of Human importin 8 (IPO8), transcript variant 1, Myc-DDK-tagged | 10 ug | | |
| RC215167L4 | Lenti ORF clone of Human importin 8 (IPO8), transcript variant 1, mGFP tagged | 10 ug | | |
| RG215167 | IPO8 (tGFP-tagged) - Human importin 8 (IPO8), transcript variant 1 | 10 ug | | |
| SC315100 | IPO8 (untagged)-Human importin 8 (IPO8), transcript variant 1 | 10 ug | | |
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.