Mouse Anti-Human IgG4 Secondary Antibody (HRP)
Cat# LS-C70328-200
Size : 200ug
Brand : LSBio
Overview
Antibody:
Human IgG4 Mouse anti-Human Monoclonal (HRP) Antibody
Application:
IHC, IHC-Fr, ELISA
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified
Specifications
Description
Human IgG4 antibody LS-C70328 is an HRP-conjugated mouse monoclonal antibody. Validated for ELISA and IHC.
Target
Human IgG4
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Horseradish Peroxidase
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Human IgG4.
Specificity
Recognizes an epitope in the pFc region of human IgG4. No cross-reactivity is observed with human IgG1, IgG2 or IgG3 isotypes.
Applications
- IHC
- IHC - Frozen (1:40 - 1:80)
- ELISA (1:25600 - 1:51200)
Usage
Suitable for use in ELISA and Immunohistochemistry. ELISA: 1:25600-1:51200. Immunohistochemistry (Frozen sections): 1:40-1:80. Positive control: Tonsil.HRP conjugates are sensitive to light. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 0.01% Thimerosal
Storage
Storage and Stability Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
tech@quimigen.pt