Applications:|Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.||Recommended Dilutions:|Western Blot: 0.5-2ug/ml|Immunohistochemistry: 5-20ug/ml|Immunocytochemistry: 5-20ug/ml|Optimal dilutions to be determined by the researcher.||Included Protein Molecular Weight Marker: |168631: Unstained Protein Molecular Weight Marker, 10-70kD|Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use.||Protein Bands: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD.|Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier.||Usage: Allow the marker to reach room temperature and mix thoroughly before use to ensure that any solids that may have precipitated at -20°C will go back into solution. Do not boil! Load the following volumes on SDS-PAGE gel:|Mini gels: 3-5ul/well|Large gel: 7ul/well|The loading volumes listed above are recommended for gels with thickness of 0.75-1mm. The loading volume should be doubled for 1.5mm thick gels.|Store at -20°C Stable for 6 months after receipt. ||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.