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> NDSB-256 [81239-45-4]

NDSB-256 [81239-45-4]

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Cat# HY-W011831-1g

Size : 1g

Brand : MedChemExpress

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NDSB-256 is a non-stain remover sulfabetaine. NDSB-256 prevents protein aggregation and promotes denaturation of chemically and thermally denatured proteins.

For research use only. We do not sell to patients.

NDSB-256 Chemical Structure

NDSB-256 Chemical Structure

CAS No. : 81239-45-4

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Description

NDSB-256 is a non-stain remover sulfabetaine. NDSB-256 prevents protein aggregation and promotes denaturation of chemically and thermally denatured proteins[1].

Molecular Weight

257.35

Formula

C12H19NO3S
CAS No.

81239-45-4
Appearance

Solid

Color

White to off-white

SMILES

O=S(CCC[N+](C)(C)CC1=CC=CC=C1)([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 175 mg/mL (680.01 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8858 mL 19.4288 mL 38.8576 mL
5 mM 0.7772 mL 3.8858 mL 7.7715 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.50%

COA (246 KB) HNMR (242 KB) LCMS (87 KB)

Handling Instructions (2659 KB)
References

  • [1]. Vuillard L, et al. Interactions of non-detergent sulfobetaines with early folding intermediates facilitate in vitro protein renaturation. Eur J Biochem. 1998 Aug 15;256(1):128-35.  [Content Brief]

  • [1]. Vuillard L, et al. Interactions of non-detergent sulfobetaines with early folding intermediates facilitate in vitro protein renaturation. Eur J Biochem. 1998 Aug 15;256(1):128-35.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.8858 mL 19.4288 mL 38.8576 mL 97.1440 mL
5 mM 0.7772 mL 3.8858 mL 7.7715 mL 19.4288 mL
10 mM 0.3886 mL 1.9429 mL 3.8858 mL 9.7144 mL
15 mM 0.2591 mL 1.2953 mL 2.5905 mL 6.4763 mL
20 mM 0.1943 mL 0.9714 mL 1.9429 mL 4.8572 mL
25 mM 0.1554 mL 0.7772 mL 1.5543 mL 3.8858 mL
30 mM 0.1295 mL 0.6476 mL 1.2953 mL 3.2381 mL
40 mM 0.0971 mL 0.4857 mL 0.9714 mL 2.4286 mL
50 mM 0.0777 mL 0.3886 mL 0.7772 mL 1.9429 mL
60 mM 0.0648 mL 0.3238 mL 0.6476 mL 1.6191 mL
80 mM 0.0486 mL 0.2429 mL 0.4857 mL 1.2143 mL
100 mM 0.0389 mL 0.1943 mL 0.3886 mL 0.9714 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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NDSB-256 Related Classifications

  • Others
  • Others
  • Biochemical Assay Reagents
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NDSB-25681239-45-43-(Benzyldimethylammonio)propane-1-sulfonate PDANDSB256NDSB 256Biochemical Assay ReagentsInhibitorinhibitorinhibit