NORMAL GOAT SERUM (NGS)
Cat# B304
Size : 10mL
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Specifications for Normal Goat Serum (NGS)
Product Details
Normal Goat Serum (NGS) - B304
blocking goat serum, blocking grade goat serum, 10% NGS, normal goat serum, goat serum blocking buffer
Goat
Target Details
Goat Serum blocking reagent or NGS was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. Normal Goat Serum was obtained from non-immunized healthy goats.
Application Details
SDS-PAGE
ChIP, IF, IHC, Multiplex - View References
Goat Serum blocking reagent or NGS has been tested by SDS-PAGE and is ideal for blocking procedures such as Western blotting, ELISA and immunochemistry to prevent nonspecific binding.
Formulation
110 mg/mL by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide and 0.01% (w/v) Gentamicin Sulfate
None
10 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store normal goat serum at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
Goat Serum blocking reagent or NGS can be used as a blocking agent to treat plastic surfaces, membrane or tissue after they have been sensitized with primary antibody or antigen. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk. It is effective in reducing nonspecific binding of proteins to reaction surfaces, thereby maximizing signal-to-noise ratio. This blocking agent is recommended for use in immunoassays where the primary antibody was produced in goat, as a source of non-specific serum protein or on tissue for immunohistochemical applications.
References (9)
Weinberg F. et al. (2020). Anti-correlation of HER2 and focal adhesion complexes in the plasma membrane. PLoS One.
Applications
IF, Confocal Microscopy PubMed
Xu NW, Dabiri JO. (2020). Low-power microelectronics embedded in live jellyfish enhance propulsion. Sci Adv.
Applications
IHC, ICC, Histology PubMed
Takizawa, D et al. (2020). Regenerative effects of human chondrocyte sheets in a xenogeneic transplantation model using immune-deficient rats. Journal of Tissue Engineering and Regenerative Medicine
Applications
IHC, ICC, Histology PubMed
Molaei M et al. (2019). NF-κB shapes metabolic adaptation by attenuating foxo-mediated lipolysis in Drosophila. Dev Cell.
Applications
ChIP PubMed
Takahashi, T et al. (2018). Rabbit xenogeneic transplantation model for evaluating human chondrocyte sheets used in articular cartilage repair. Journal of Tissue Engineering and Regenerative Medicine
Applications
IHC, ICC, Histology PubMed
Ghitani N, Barik A, Szczot M, et al. (2017). Specialized Mechanosensory Nociceptors Mediating Rapid Responses to Hair Pull. Neuron.
Applications
IF, Confocal Microscopy PubMed
Shah SH et al. (2015). Hierarchical paracrine interaction of breast cancer associated fibroblasts with cancer cells via hMAPK-microRNAs to drive ER-negative breast cancer phenotype. Cancer Biol Ther.
Applications
IF, Confocal Microscopy PubMed
Almanaa, TN et al. (2013). A new method for identifying stem-like cells in esophageal cancer cell lines. Journal of Cancer
Applications
IHC, ICC, Histology PubMed
Papapattu GS et al. (2009). Selective expression of CD44, a putative prostate cancer stem cell marker, in neuroendocrine tumor cells of human prostate cancer. Prostate.
Applications
IHC, ICC, Histology; Multiplex Assay PubMed
Related Protocols
Adherent Cell Lysis Protocol
Bifunctional Cross-Linking Protocol
Chromatin Immunoprecipitation (ChIP) Protocol
Heat-induced Antigen Retrieval Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
IP-WB with TrueBlot® Protocol
Nucleosome ChIP Protocol
Protease-induced Antigen Retrieval Protocol
Re-ChIP Protocol
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Related Resources
Assay Substrate Selection Guide
Articles
View Article
Blocking Buffer Selection Guide
Articles
View Article
Streptavidin: Properties and Applications
Articles
View Article