PCNA (3G1) Monoclonal Antibody, APC-Cy7 Conjugated
Cat# bsm-33035M-APC-Cy7
Size : 100ul
Brand : Bioss
PCNA (3G1) Monoclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Overview | |
Catalog # | bsm-33035M-APC-Cy7 |
Product Name | PCNA (3G1) Monoclonal Antibody, APC-Cy7 Conjugated |
Applications | WB, IF(IHC-P) |
Reactivity | Human, Mouse, Rat |
Specifications | |
Conjugation | APC-Cy7 |
Host | Mouse |
Source | Recombinant human PCNA protein |
Clonality | Monoclonal |
Clone # | 3G1 |
Isotype | IgG |
Concentration | 1ug/ul |
Purification | Purified by Protein G. |
Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
Target | |
Gene ID | 5111 |
Swiss Prot | P12004 |
Synonyms | Proliferating cell nuclear antigen; PCNA; Cyclin |
Background | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. |
Application Dilution | |
WB | 1:300-5000 |
IF(IHC-P) | 1:50-200 |