Polymyxin B mouse monoclonal antibody, clone 45, Low Endotoxin
Specifications
Product Data
Clone Name
45
Application
ELISA, WB
Recommended Dilution
Immunoassays. Western blot: The typical starting working dilution is 1/10.
Reactivity
Human
Antibody Host
Mouse
Isotype
IgM
Clonality
Monoclonal
Specificity
The monoclonal antibody 45 reacts with Polymyxin B. It enables the possibilities to study quantitatively the interaction of PMB and LPS.
Buffer
State: Low Endotoxin State: Liquid Culture Medium with a Low endotoxin Level Preservative: 0.02% Sodium Azide
Concentration
lot specific
Conjugation
Unconjugated
Storage
Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability
Shelf life: one year from despatch.
Shipping
Blue Ice
Background
The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
