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Western Blot (Cell lysate)
Note

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Application Data with Unconjugated Antibody

Western Blot (Cell lysate)

Western blot analysis of human cell line mix with RAB5A polyclonal antibody (PAB29000) at 1:1000 dilution.

Immunofluorescence
Note

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Application Data with Unconjugated Antibody

Immunofluorescence

Immunofluorescent staining of Bouin's fixed paraffin-embedded backskin sections of transgenic mice with RAB5A polyclonal antibody (PAB29000).

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic peptide of RAB5A.

    Immunogen

    A synthetic peptide conjugated with KLH corresponding to human RAB5A.

    Host

    Rabbit

    Reactivity

    Bovine, Human, Monkey, Mouse, Rat

    Specificity

    The antibody recognizes 26 kDa Rab5 of the above species origins.

    Form

    Liquid

    Conjugation

    ATTO 488

    Purification

    Protein A purification

    Recommend Usage

    Immunocytochemistry/Immunofluorescence (1:80)
    Immunohistochemistry (1:100)
    Western blot (1:1000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 0.09% sodium azide).

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Application Data with Unconjugated Antibody

    Western Blot (Cell lysate)

    Western blot analysis of human cell line mix with RAB5A polyclonal antibody (PAB29000) at 1:1000 dilution.

    Immunofluorescence

    Immunofluorescent staining of Bouin's fixed paraffin-embedded backskin sections of transgenic mice with RAB5A polyclonal antibody (PAB29000).

  • Applications

    Western Blot

    Immunohistochemistry

    Immunofluorescence

  • Gene Info — RAB5A

    Entrez GeneID

    5868

    Protein Accession#

    Q6FI44

    Gene Name

    RAB5A

    Gene Alias

    RAB5

    Gene Description

    RAB5A, member RAS oncogene family

    Omim ID

    179512

    Gene Ontology

    Hyperlink

    Gene Summary

    O

    Other Designations

    RAS-associated protein RAB5A

  • Interactomes
  • Pathways
  • Diseases