PAB10666 | Rabbit Anti-Horse IgG F(ab')2 secondary antibody (Rhodamine)

Specifications

Product Description

Rabbit anti-horse IgG recognizes horse IgG F(ab')2 fragment. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Rhodamine.PAB,polyclonal Ab,polyclonal antibody

Immunogen

Horse IgG F(ab')2 fragment

Host

Rabbit

Reactivity

Horse

Specificity

No reaction was observed against horse IgG F(c).

Form

Lyophilized

Conjugation

Rhodamine (TRITC)

Purification

This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.

Conjugation Note

3.6 moles TRITC per mole of rabbit IgG

Recommend Usage

The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from 20 mM KH₂PO₄, 150 mM NaCl, pH 7.2 (0.01% sodium azide)

Storage Instruction

Store at 4°C on dry atmosphere.
After reconstitution with 1 mL of deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.

Note

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications

Immunofluorescence
Publication Reference
- Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies.
  J A Titus, R Haugland, S O Sharrow, D M Segal.
  
  Journal of Immunological Methods 1982 Apr; 50(2):193.

Rabbit Anti-Horse IgG F(ab')2 secondary antibody (Rhodamine)