Rabbit polyclonal antibody to eIF2C2

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Cat# CQA3218-200ul

Size : 200ul

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Phone : +1 850 650 7790

Brand : Cohesion Biosciences

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Phone : +1 850 650 7790

Western blot analysis of eIF2C2 expression in Jurkat (A) whole cell lysates. (Predicted band size: 93; 97 kD; Observed band size: 110 kD)
Immunofluorescent analysis of eIF2C2 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Name:	Anti-eIF2C2 Antibody
Cat No:	CQA3218
Source:	Rabbit
Reactivity:	H, M, R
Applications:	WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to eIF2C2

Immunogen：Recombinant full length protein of human eIF2C2

Purification：The antibody was purified by immunogen affinity chromatography.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)

Gene Symbol：AGO2

Alternative Names：EIF2C2; Protein argonaute-2; Argonaute2; hAgo2; Argonaute RISC catalytic component 2; Eukaryotic translation initiation factor 2C 2; eIF-2C 2; eIF2C 2; PAZ Piwi domain protein; PPD; Protein slicer

Entrez Gene (Human)： 27161;

Entrez Gene (Mouse)： 239528;

Entrez Gene (Rat)： 59117;

SwissProt (Human)： Q9UKV8;

SwissProt (Mouse)： Q8CJG0;

SwissProt (Rat)： Q9QZ81;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.