Rat Anti-Mouse Kappa-BIOT

Cat# 1180-08

Size : 0.5mg

Brand : Southern Biotech

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Clone H139-52.1
Isotype Rat (LOU/Ws1) IgG1κ
Isotype Control Rat IgG1-BIOT (KLH/G1-2-2)
Specificity Mouse Kappa
Conjugate BIOT (Biotin)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications Flow Cytometry – Quality tested 2,5-8
ELISA – Quality tested 2-4
FLISA – Quality tested
Immunocytochemistry – Reported in literature 8
Western Blot 9

RRID Number AB_2794681
Gene ID 243469 (Mouse)
Gene ID Symbol Igk (Mouse)
Gene ID Aliases kappa
UniProt ID P01837 (Mouse
UniProt Name IGKC_MOUSE (Mouse)
Documentation
Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

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  1. 1. Labit C, Pierres M. Rat monoclonal antibodies to mouse IgG1, IgG2a, IgG2b, and IgG3 subclasses, and kappa chain isotypic determinants. Hybridoma. 1984;3:163-9. (Immunogen)
  2. 2. Mitchell DA, Pickering MC, Warren J, Fossati-Jimack L, Cortes-Hernandez J, Cook HT, et al. C1q deficiency and autoimmunity: the effects of genetic background on disease expression. J Immunol. 2002;168:2538-43. (ELISA, FC)
  3. 3. Kamala T. Hock immunization: A humane alternative to mouse footpad injections. J Immunol Methods. 2007;328:204-14. (ELISA)
  4. 4. Heidari Y, Fossati-Jimack L, Carlucci F, Walport MJ, Cook HT, Botto M. A lupus-susceptibility C57BL/6 locus on chromosome 3 (Sle18) contributes to autoantibody production in 129 mice. Genes Immun. 2009;10:47-55. (ELISA)
  5. 5. Pathak S, Ma S, Trinh L, Lu R. A role for interferon regulatory factor 4 in receptor editing. Mol Cell Biol. 2008;28:2815-24. (FC)
  6. 6. Ma S, Pathak S, Trinh L, Lu R. Interferon regulatory factors 4 and 8 induce the expression of Ikaros and Aiolos to down-regulate pre-B-cell receptor and promote cell-cycle withdrawal in pre-B-cell development. Blood. 2008;111:1396-403. (FC)
  7. 7. Ma S, Pathak S, Mandal M, Trinh L, Clark MR, Lu R. Ikaros and Aiolos inhibit pre-B-cell proliferation by directly suppressing c-Myc expression. Mol Cell Biol. 2010;30:4149-58. (FC)
  8. 8. Soni C, Wong EB, Domeier PP, Khan TN, Satoh T, Akira S, et al. B cell-intrinsic TLR7 signaling is essential for the development of spontaneous germinal centers. J Immunol. 2014;193:4400-14. (ICC)
  9. 9. SouthernBiotech unpublished data (WB)

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