Rat IgG2a Antibody : APC/CY5.5

Cat# OASB00111

Size : 0.1mg

Brand : Aviva Systems Biology

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Rat IgG2a Antibody : APC/CY5.5 (OASB00111)

Datasheets/ManualsPrintable datasheet for Rat IgG2a Antibody : APC/CY5.5 (OASB00111)
Product Info
ClonalityMonoclonal
CloneKLH/G2a-1-1
IsotypeIgG2a
HostRat
ConjugationAPC|Cyanine 5.5
ApplicationFC
Reconstitution and StorageStore at 2-8C
Concentration0.1 mg/mL
CharacterizationTo insure lot- to- lot consistency, each batch of product is tested to conform with characteristics of a standardreference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScanTM flowcytometer.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionFlow Cytometry: Fluorescein conjugate
Biotin conjugate
R-phycoerythrin conjugate
R-phycoerythrin conjugate-Texas Red®
Allophycocyanin conjugate
Spectral Red conjugate
Cyanine 5 conjugate
PE/CY5.5 and PE/CY7 conjugates
APC/CY5.5 and APC/CY7 conjugates
AF488 and AF647 conjugates
<=1 ug/106 cells
<=1 ug/106 cells
<=0.2 ug/106 cells
<=0.2 ug/106 cells
<=0.2 ug/106 cells
<=0.2 ug/106 cells
<=0.2 ug/106 cells
<=0.2 ug/106 cells
<=0.2 ug/106 cells
<=0.2 ug/106 cells
Application InfoFlow cytometry, Enzyme-Linked-Immunosorbent-Assay (ELISA)
Other Applications DataSince applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs.
Storage- The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
- The Alexa Fluor 488 (AF488) and Alexa Fluor 647 (AF647) conjugates are supplied as 0.1mg in 0.2mLPBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD), R- phycoerythrin - Texas Red (R- PE- TXRD), R- phycoerythrin- Cyanine 5.5(R- PE- CY 5.5), R- phycoerythrin- Cyanine 7 (R- PE- CY 7), Allophycocyanin- Cyanine 5.5 (APC- CY 5.5)and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C
- The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C
- With the exception of the low endotoxin, azide- free product, the vials should be stored at 2- 8 C
Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquotand freeze the low endotoxin, azide- free product at - 20 C immediately upon receipt. Each reagent isstable for the period shown on the bottle label if stored as directed.
Gene Full NameIg gamma-2 chain C region