Recombinant mouse TNF-alpha protein

Cat No.
ATGP3184

Size

QTY

List




Product Information

Product Type
Recombinant Protein
Expression System
Baculovirus
Domain
80-235aa
UniProt No.
P06804
NCBI Accession No.
NP_038721
Alternative names
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Product Specification

Molecular Weight
18kDa (162aa)
Concentration
1mg/ml (determined by absorbance at 280nm)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.02ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Biological Activity

Mouse TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Related Publications

    Kim MJ, et al. Paeonia lactiflora Root Extract and Its Components Reduce Biomarkers of Early Atherosclerosis via Anti-Inflammatory and Antioxidant Effects In Vitro and In Vivo. (Antioxidants (Basel). 2021)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.