Reduced Glutathione (GSH) Colorimetric Assay Kit

Cat# E-BC-K030-M-500

Size : 500Assays

Brand : Elabscience

Contact local distributor :


Phone : +1 850 650 7790

Detection Principle Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly.
Synonyms GSH
Sample Type Serum,Plasma,Cell culture supernatant,Tissue,Cell
Detection Method Colorimetric method
Detection Instrument Microplate reader (405-414 nm)
Research Area Oxidative Stress ,  Cuproptosis ,  Ferroptosis
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 2 μmol/L
Detection Range 2-100 μmol/L
Precision inter-assay CV: 3.2% | intra-assay CV: 1.9%
Assay Time 25 min
Cat.No. Product Name Sizes
E-BC-K096-M Glutathione Peroxidase (GSH-Px) Activity Assay Kit 96T , 48T
E-BC-K097-M Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit 96T
E-BC-K099-S Glutathione Reductase (GR) Activity Assay Kit 100Assays , 50Assays
E-BC-K278-S Glutathione-S-Transferase (GST) Activity Assay Kit 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
Human plasma 1
10% Mouse brain tissue homogenization 1
10% Mouse liver tissue homogenization 1
Hela cell homogenization (0.999 mgprot/mL) 1
Rat serum 1
Rat plasma 1
Mouse serum 1
10% Carrot tissue homogenization 1
293T supernatant 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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