Reduced Glutathione (GSH) Colorimetric Assay Kit
Cat# E-BC-K030-M-500
Size : 500Assays
Brand : Elabscience
Detection Principle | Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly. |
Synonyms | GSH |
Sample Type | Serum,Plasma,Cell culture supernatant,Tissue,Cell |
Detection Method | Colorimetric method |
Detection Instrument | Microplate reader (405-414 nm) |
Research Area | Oxidative Stress , Cuproptosis , Ferroptosis |
Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
Storage | This product can be stored at 2-8°C for 12 months with shading light. |
Valid Period | 12 months |
Sensitivity | 2 μmol/L |
Detection Range | 2-100 μmol/L |
Precision | inter-assay CV: 3.2% | intra-assay CV: 1.9% |
Assay Time | 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
Sample Type | Dilution Factor |
---|---|
Human serum | 1 |
Human plasma | 1 |
10% Mouse brain tissue homogenization | 1 |
10% Mouse liver tissue homogenization | 1 |
Hela cell homogenization (0.999 mgprot/mL) | 1 |
Rat serum | 1 |
Rat plasma | 1 |
Mouse serum | 1 |
10% Carrot tissue homogenization | 1 |
293T supernatant | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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